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　　名稱：抗Visfatin抗體(anti-Visfatin antibody)

　　貨號(hào)：FNab09847

　　適應(yīng)種屬：人源，大鼠，小鼠

　　規(guī)格：100ug(微克)

　　狀態(tài)：液體

　　純化方法：蛋白A/G柱純化

　　純度：經(jīng)sds-page分析，純度大于95

　　宿主：小鼠

　　亞型：IgG2b

　　存儲(chǔ)：用添加了0.02疊氮化鈉和50甘油的pbs溶解，ph值7.3，可在-20℃下保存12個(gè)月（避免反復(fù)凍融）

　　免疫原：nicotinamide phosphoribosyltransferase

　　免疫原別稱：1110035O14Rik, DKFZp666B131, NAmPRTase, NAMPT, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN

　　分子量：52 kDa

　　驗(yàn)證應(yīng)用：ELISA, WB, IHC, IF

　　建議稀釋比例：WB: 1:1000-1:4000; IHC: 1:50-1:500; IF: 1:50-1:500

　　克隆性：?jiǎn)慰寺?/p>

　　uniprot id：P43490

　　背景介紹：Nicotinamide phosphoribosyltransferase (NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM) , and it is now evidenced that NAMPT may plays a role in lipid metabolism。

　　Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab09847 (Visfatin antibody) at dilution of 1:200。

　　Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab09847( Visfatin antibody ) at dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H L)。

　　rat heart tissue were subjected to SDS PAGE followed by western blot with FNab09847 (Visfatin antibody) at dilution of 1:2000。